Many biospecimen repositories or tissue core facilities collect de-identified biospecimens such as blood or tissue from human patients for use in IRB approved research projects studying everything from factors that contribute to cancer to studying specific disorders such as Crohn's disease, diabetes, or Alzheimer's disease.
Contact Us today to find out how we can help you with the quality control testing of your human biospecimens.
There are many ways in which STR profiling can be utilized as an essential quality control check of human biospecimens, here are a few examples:
STR profiling is the most often used method for human DNA identification – it is the same technique that is recommended for the authentication of human cell lines used for research purposes . The STR profiling method is quick, cost-efficient, and the human identification results are straightforward (only identical twins have the same STR profile). Once you receive your baseline reference STR profile of your de-identified patient samples, you can use that reference STR profile as a QC tracking code for all downstream uses of those specimens (e.g., in the creation of primary and/or cancer cell lines, stem cell lines or xenografts, or in translational genomics research between the normal and tumor/diseased tissue).
Inadvertent cell line contamination has been a major problem for biomedical researchers for over 50 years ; by performing this essential quality control check on the biospecimens used for translational genomics research or used to create cancer cell lines, stem cell lines and xenografts, you are helping to keep research on a solid foundation.
The advantages of STR profiling over other “identification” methods are, at minimum, twofold:
The turnaround time for our STR profiling test for human biospecimens is as quick as 1-2 business days and it includes 15 autosomal sites plus amelogenin (gender marker) using the PowerPlex16HS kit (Promega). The test also includes a MOUSE marker for the detection of mouse DNA contamination, especially helpful for patient-derived xenograft (PDx) models and labs that utilize both human and mouse cell lines (to exclude cross-contamination). This essential quality control test is cost efficient and easy to interpret with low prices (volume discounts available). See our pricing options.
*Please note that FFPE samples or other non-standard samples (e.g., bone) will incur additional DNA extraction charges above what is charged for fresh tissue or blood DNA extractions and may take longer to process due to the condition of the non-standard biospecimen.
Please note: STR profiling is for research purposes only